Posts tagged "microscopy"
Drosophila Heart by Girish C. Melkani
"Amyloid-like inclusions have been associated with Huntington’s disease, and patients exhibit a high incidence of cardiovascular events. Melkani and colleagues generated a Drosophila (fruit fly) model of cardiac amyloidosis. It displays accumulation of mutant Huntingtin aggregates and oxidative stress in myocardial cells upon heart-specific expression of Huntingtin protein fragments with disease-causing poly-glutamine repeats. Using genetic manipulation, the authors showed that modulation of both protein unfolding, and oxidative stress pathways, is required to ameliorate the detrimental mutant Huntingtin defects. The image shows reduced and disorganized myosin- (pink) and actin- (cyan) containing myofibrils along with mutant Huntingtin positive aggregates (green) in the heart."
Confocal laser scanning microscopy of rat kidney.
Tiles, stitched together with ZEN software to generate hi-res image.
Mr. Brent Bill
Burnsville, MN, USA
Specimen: Zebrafish Embryo
Echiniscus mediantus (tardigrade, water bear), in various states of movement
Technique : Darkfield
Mikrobiologische Vereinigung München e.V. (MVM) München, Bavaria, Germany
Primary mammary epithelium in 3D Matrigel culture.
The mammary gland, which produces milk, is composed of a dense network of ducts that gradually forms after birth through a complex process of budding, invasion, and branching. At birth, the system is a rudimentary duct tree, but in response to hormones, cells in the ducts proliferate and migrate, allowing the ducts to elongate and invade through the mesenchyme layer of mammary tissue into the mammary fat pad, where they then begin to branch. Puberty brings further structural changes to the system of ducts, whereas pregnancy induces the formation of alveolar structures within the ducts for milk production.
Credit: Andrew J. Ewald, Johns Hopkins University.
Taking out the defender
The in vivo interaction between a Pseudomonas aeruginosa biofilm, on a silicone implant, and the responding polymorphonuclear leukocytes.
Image: SEM imaging depicts the interaction at day 1 post insertion of the implant in the peritoneal cavity of a mouse. The leukocytes (yellow) are damaged with obvious cavities in the cell membrane and killed by the bacteria (cyan) following contact with the biofilm. The SEM image was pseudo colored in Photoshop CS5 using a Wacom Cintiq 24HD, by Michael Larsen.
Click here to learn more about scanning electron microscopy (SEM) by Carl Zeiss.
Nikon Small World Contest 2013
Honorable Mention: A 200x view of the radula (rasping organ) of the mollusc Buccinum undatum (Common Whelk), by Dr. David Maitland, from Feltwell, Norfolk, UK. (Dr. David Maitland)
Pearceite, an uncommon silver mineral, in beautiful hexagonal crystals, from a copper mine in Spain. Reflected Light, Stereomicroscopy at 100X
An oriens-lacunosum moleculare interneuron in the hippocampus labeled with Alexa 488.
Credit: Michael N. Economo.
APOPTOSIS: Programmed Cell Death
"Remodeling To Die For"
Electron microscopy by L. Scorrano
Dept. of Cell Physiology and Metabolism
University of Geneva
- The release of cytochrome c from mitochondria into the cytosol triggers a chain reaction that drives cells towards apoptosis.
- To facilitate this release, the inner mitochondrial membranes (green) fuse and remodel.
Image: Electron tomograms contrast the structural differences between mitochondria under normal (left) and apoptotic (right) conditions.
The cristae are pseudo-colored in green;
the inner boundary membrane is in white,
and the outer membrane is in red.
"Death Most Beautiful"
Multiphoton fluorescence microscopy
by Thomas Deerinck and Mark Ellisman [NCMIR, UCSD]
IMAGE: an apoptotic HeLa cell (middle) among non-dying neighbors.
Golgi apparatus (yellow)
cell nuclei (blue).
13 October 2013
Sniffing Out Glue
Our bodies are held together by a sort of molecular glue called collagen. It’s the most common protein in the body, and forms tough fibres that hold our innards in place and help everything from organ growth to cell movement. But how this ever-abundant protein is replenished presents something of a gap in our knowledge. Scientists took advantage of recent advances in microscope technology to take a new look at the problem, watching what happens when collagen (white strands pictured) is introduced to skin. They could see how different parts of cells (labeled in red, blue and green) dealt with the influx, and identified the ways they break it down. This is an important revelation as too much collagen can lead to fibrosis, too little can cause osteoporosis and osteoarthritis, and collagen control is central to the strategy cancer cells employ to spread around the body.
Written by Anthony Lewis
Thomas H. Bugge
National Institutes of Health, Bethesda,
Originally published under a Creative Commons Attribution license
Published in J. Cell Biol.